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  • Article title
  • Abstract
  • Key words
  • Introduction
  • Fungal-derived natural product discovery – methodologies from the past to the future
    • Reflections of the past – Seeing is believing
    • Recent advances – Technical innovations driving modern natural product discovery
  • Workflow – from the fungus to the compound
    • Sources of novel metabolites and the importance of taxonomy
    • Structure elucidation of novel metabolites and screening libraries
    • Bioreactor process development for promising candidates
  • Biological aspects
    • Classical fermentation experiments – One strain, many compounds
    • Regulation and appearance of secondary metabolites encoding gene clusters in fungi
    • Ecological context of secondary metabolites produced by fungi
    • Novel fungi and novel habitats lead to novel chemistry
    • Notable examples from Ascomycota and Basidiomycota
    • Secondary metabolites as taxonomical tools in the systematics of fungi
    • Secondary metabolites as biochemical tools
  • Synthetic biology approaches to natural product chemistry
  • Exploiting the biosynthetic machinery to increase chemical space: Mutasynthesis and rational design
  • Outlook
  • Acknowledgements
  • Additional information
    • Conflict of interest
    • Ethical statement
    • Adherence to national and international regulations
    • Funding
    • Author contributions
    • Author ORCIDs
    • Data availability
  • References
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