Pan-genome analysis of F. pseudograminearum: A proportion of genes in the core, dispensable and private genomes; B estimation of F. pseudograminearum core genome. Parameter κc is the amplitude of the exponential decay, τc is the decay constant and Ω is the best-fit value of the core genome; C estimation of the pan-genome size of F. pseudograminearum. The curve is a least-squares fit of the power law to the median; D estimation of new genes: The number of individual-specific genes is plotted as a function of the number of genomes sequentially added. Parameters κs and τs are equivalent to κc and τc, respectively and tg(θ) measures the best-fit number of specific genes; E a SplitsTree network showing relationships of all F. pseudograminearum isolates with no outgroup, which was estimated using the NeighborNet method using pairwise genetic distances (F84 distances obtained from the concatenated 5,757 orthologs aligned in codons).