Manual curation of the PO212 and S27 assemblies: A whole-genome dot-plot between P. rubens strains 212 (PO212) and S27 using version 1 of the assemblies before manual curation. The PO212 assembly (x-axis) was utilised as the reference. Green circles mark the start of scaffold 1. Red circles indicate the start of the scaffolds designed for merging. The blue boxes on the x- and y-axis correspond to the following: *1: S27 scaffolds ordered from 9 to 20 and *2: PO212 scaffolds ordered from 9 to 23; B scheme of the overlapping region between the end of one scaffold and the start of another, together with the position of the designed oligonucleotides; C PCR product to verify the continuity of the regions in the PO212 and S27 strains. P indicates PO212 genomic DNA and S indicates S27 genomic DNA. The order from left to right is PO212 (2+10 = 5.5 kb, 1+11 = 5.3 kb, 13+14 = 4 kb, 6+8 = 4.3 kb, 7+13 = 4.9 kb, 9+16 = 5 kb, 3+17 = 4.9 kb, 4+15 = 2.3 kb, 12+6+8 = 5.6 kb and 1+11+3+17 = 5.5 kb). S27 (14+13 = 3.2 kb, 4+5 = 4.2 kb, 15+2 = 4.2 kb, 1+9 = 6.7 kb and 9+11 = 5.3 kb). Mw: Molecular weight marker. The black arrows indicate the band sizes of 5, 3 and 2 kb from top to bottom; D whole-genome dot-plot between strains PO212 and S27 using version 2 of assemblies after manual curation. The PO212 assembly (x-axis) was utilised as a reference. The green circle indicates the start of scaffold 1 in both genomes. The blue boxes on the x- and y-axis correspond to the following: *3: S27 scaffolds 7, 10, 11, 12 and 13 and *4: PO212 scaffolds 7, 8, 9, 10, 11, 12 and 13.