Synteny between the P2niaD18 genome (left) and the PO212 genome (right) (A, C) and between the Wisconsin 54-1255 genome (left) and the PO212 genome (right) (B, D): A whole-genome dot-plot between P. rubens strain 212 (PO212) and P2niaD18 genome (GCA_000710275.1). The PO212 assembly (x-axis) was utilised as a reference; B whole-genome dot-plot between P. rubens strain 212 (PO212) and Wisconsin 54-1255 genome (GCA_000226395.1). The PO212 assembly (x-axis) was utilised as a reference; C scaffold ordering of the P2niaD18 genome (left) to the scaffolds of the PO212 genome (right); D scaffold ordering of the Wisconsin 54-1255 genome (left) to the scaffolds of the PO212 genome (right). The PO212 scaffolds are illustrated on the right in 13 different colours, with the labelling corresponding to that employed in the genome assembly. The grey bars correspond to a track showing the number of repetitive elements in 120 kb ranges. On the left, the scaffolds of P2niaD18 (C) and Wisconsin 54-1255 (D) are linked to the PO212 scaffolds by lines representing regions of high sequence similarity. Numerical labels indicate the nucleotide of the break junction between rearranged regions.