A Phylogenetic relationship of the genes encoding mutanases from PO212 and P2niaD18 using MEGA11. The phylogenetic tree was built using six mutanases encoding genes from PO212 (PO212g058370, PO212g085410, PO212g101870, PO212g096930, PO212g013760 and PO212g033560) (red) and the corresponding genes encoding mutanases from P2niaD18 (KZN91491, KZN92410, KZN92305 and KZN94461) (green). The sequence of a glycosyl transferase was utilised as a root (PO212g014370) (purple). Bootstrap values are indicated adjacent to the nodes. The tree was inferred by Maximum Likelihood. The tree has been drawn to scale, with branch lengths measured as the number of substitutions per site. The codon positions encompass in the analysis included first+second+third+non-coding positions. The final dataset comprised 4060 positions; B schematic representation of PO212g085410 mutanase and the homologue in P2niaD18 (KZN92410); C PO212g101870 mutanase and the homologue region in P2niaD18; D PO212g096930 mutanase and the homologue in P2niaD18 (KZN92305); E PO212g013760 mutanase and the homologue region in P2niaD18; F PO212g033560 and the homologue in P2niaD18 (KZN94461); G PO212g058370 and the homologue in P2niaD18 (KZN91491). The flanking and homologous genes between PO212 and P2niaD18 are coloured in grey. Red colour indicates PO212 mutanases. Blue colouration indicates the single genes for PO212. P2niaD18 mutanases are represented in green colour. The genes that are unique to P2niaD18 are shown in yellow. The prefix PO212 has been removed from the nomenclature of the genes in order to facilitate the legibility of the figure. The genes are to be initiated with the letter “g”.