Identification of Numts in Penicillium rubens: A PO212 and S27 raw reads were mapped to the PO212 genome in scaffold 2 between coordinates 4,488,480–4,498,480; B reads from PO212 and S27 sequencing that did not map to the mitochondrial genome of P2niaD18, were mapped to the PO212 genome in scaffold 2 between coordinates 4,488,480–4,498,480; C schematic representation illustrates the oligonucleotides mapping and predicted amplicon size. Green circles indicate the gene number: 1- PO212g047600, 2- PO212g047610, 3- PO212g047620, 4- PO212g047630. IGV images; D the PCR product of PCR 1 (Mit 1F and Mit 1R) and PCR 2 (Mit 2F and Mit 2R). The amplified fragment is 4.3 kb for PCR 1 and 2 kb for PCR 2, corresponding to the predicted size of the amplicon in both strains, PO212 and S27. C- Corresponds to the negative control of the PCR. Mw: Molecular weight marker; E PCR product of PCR 3 (Mit 1F and Mit 2R) and PCR 4 (same oligonucleotides as PCR 3). The amplified fragment for PCR 3 is greater than 10 kb for both strains, PO212 and S27. No PCR product was detected in PCR 4. C- Corresponds to the negative control of the PCR. Mw: Molecular weight marker; F PCR product of PCR 3 in other P. rubens DNA strains, where an amplicon greater than 10 kb can be observed for all strains except CH5 (2.5 kb).