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  • ContentsContents
  • Article InfoArticle Info
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  • Inst. CodeInst. Code
  • Article title
  • Abstract
  • Key words
  • Introduction
  • Materials and methods
    • Strains and morphological study
    • DNA extraction, PCR amplification and sequencing
    • Molecular phylogenetic analysis of Nigromargarita tarda
    • RNA extraction and cDNA amplification
    • Dataset preparation for intron analysis
    • The co-phylogeny analysis and ancestral character reconstructions for introns
  • Results
    • Phylogenetic analysis
    • Taxonomy
    • The introns Nta.18SS453 and Pcl Ple.18S1
    • The spread of Pcl Ple.18S1 within Pleosporales
  • Discussion
    • Morphological uniqueness of Nigromargarita tarda
    • The horizontal transmission of Ple.18S1 introns
    • The vertical transmission and loss of Ple.18S1 introns
    • Host range restriction of intron Pcl Ple.18S1
  • Conclusion
  • Acknowledgements
  • Additional information
    • Conflict of interest
    • Ethical statement
    • Adherence to national and international regulations
    • Funding
    • Author contributions
    • Author ORCIDs
    • Data availability
  • References
  • Supplementary materials
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