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  • ContentsContents
  • Article InfoArticle Info
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  • Article title
  • Abstract
  • Key words
  • Introduction
  • Materials and methods
    • Sample collection
    • DNA extraction
    • Design of primers and crRNA
    • RPA assay optimization
    • CRISPR/Cas12a-FRB detection
    • CRISPR/Cas12a-PLFS detection
    • RPA-CRISPR/Cas12a for detecting pathogens in plant samples
  • Results
    • Specificity of RPA and RPA-CRISPR/Cas12a primers and crRNA
    • Optimization of RPA reaction time and temperature
    • Optimization of CRISPR/Cas12a-FRB assay
    • Optimization of CRISPR/Cas12a-PLFS assay
    • Sensitivity of RPA-CRISPR/Cas12a assay
    • Application of RPA-CRISPR/Cas12a assay in simulated plant samples
  • Discussion
  • Acknowledgements
  • Additional information
    • Conflict of interest
    • Ethical statement
    • Adherence to national and international regulations
    • Funding
    • Author contributions
    • Author ORCIDs
    • Data availability
  • References
  • Supplementary material
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