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Article title
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Abstract
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Key words
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Introduction
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Materials and methods
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Culture and DNA collection
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DNA extraction
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PCR amplification
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Amplicon sequencing
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Data processing
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1. Long-read processing
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2. Peak detection
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3. Denoising
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4. Variant & SNP detection
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Multiple sequence alignment
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Phylogenetic analysis
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Quality control
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Ribosomal operon variants
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Statistics with ribosomal coding and non-coding regions
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Results and discussion
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A comprehensive database for the rrn operon in the Neocallimastigomycota
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Variability within the rrn operon
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Number of rrn operon sequence variants
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Quantifying sequence variability in the rrn operon
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Phylogenetic analysis of the Neocallimastigomycota using the rrn operon
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The case of Caecomyces and Cyllamyces
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Identification of new barcodes in the Neocallimastigomycota rrn operon
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Family-level resolution of the Neocallimastigomycota rrn operon
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Conclusions
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Acknowledgments
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References
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Additional information
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Conflict of interest
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Ethical statement
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Artificial Intelligence (AI) use
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Adherence to national and international regulations
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Funding
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Author contributions
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Author ORCIDs
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Data availability
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Supplementary material
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