IMA Fungus 2(2): 177-189, doi: 10.5598/imafungus.2011.02.02.09
Molecular techniques for pathogen identification and fungus detection in the environment
Kin-Ming Tsui, James Woodhall‡, Wen Chen§, C. André Lévesque|, Anna Lau¶, Cor D. Schoen#, Christiane Baschien¤, Mohammad J. Najafzadeh«, G. Sybren De Hoog«‡ Sand Hutton, The Food and Environment Research Agency, York§ Agriculture and Agri-Food Canada, Central Experimental Farm, Ottawa, Canada| Agriculture and Agri-Food Canada, National Mycological Herbarium, Ottawa, Canada¶ Centre for Infectious Diseases and Microbiology and the University of Sydney, Westmead Hospital, Westmead, Australia# Business Unit Bio-Interactions and Plant Health, Plant Research International, Wageningen¤ Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Department of Bioresources for Bioeconomy and Health Research, Brunswick, Germany« CBS-KNAW Fungal Biodiversity Centre, Utrecht
© Kin-Ming Tsui, James Woodhall, Wen Chen, C. Lévesque, Anna Lau, Cor Schoen, Christiane Baschien, Mohammad Najafzadeh, G. De Hoog. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY-NC 4.0), which permits to copy and distribute the article for non-commercial purposes, provided that the article is not altered or modified and the original author and source are credited. Citation:
Tsui K-M, Woodhall J, Chen W, Lévesque CA, Lau A, Schoen CD, Baschien C, Najafzadeh MJ, De Hoog GS (2011) Molecular techniques for pathogen identification and fungus detection in the environment. IMA Fungus 2(2): 177-189. https://doi.org/10.5598/imafungus.2011.02.02.09 |  |
Abstract
Many species of fungi can cause disease in plants, animals and humans. Accurate and robust detection and quantification of fungi is essential for diagnosis, modeling and surveillance. Also direct detection of fungi enables a deeper understanding of natural microbial communities, particularly as a great many fungi are difficult or impossible to cultivate. In the last decade, effective amplification platforms, probe development and various quantitative PCR technologies have revolutionized research on fungal detection and identification. Examples of the latest technology in fungal detection and differentiation are discussed here.
Keywords
FISH, LAMP, macroarray, medical mycology, molecular diagnostics, molecular ecology, padlock probe, pathogenic fungi, plant pathology, rolling circle amplification