IMA Fungus 4(2): 161-167, doi: 10.5598/imafungus.2013.04.02.01
Loss of arbuscular mycorrhizal fungal diversity in trap cultures during long-term subculturing
Dora Trejo-Aguilar‡, Liliana Lara-Capistrán‡, Ignacio E. Maldonado-Mendoza§, Ramón Zulueta-Rodríguez‡, Wendy Sangabriel-Conde‡, María Elena Mancera-López§, Simoneta Negrete-Yankelevich|, Isabelle Barois|‡ Universidad Veracruzana., Laboratorio de organismos benéficos. Facultad de Ciencias Agrícolas., Xalapa§ Departamento de Biotecnología Agrícola., Instituto Politécnico Nacional (IPN). CIIDIR-Sinaloa., Guasave| Instituto de Ecología A. C., Red de Ecología Funcional., Xalapa
© Dora Trejo-Aguilar, Liliana Lara-Capistrán, Ignacio Maldonado-Mendoza, Ramón Zulueta-Rodríguez, Wendy Sangabriel-Conde, María Mancera-López, Simoneta Negrete-Yankelevich, Isabelle Barois. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY-NC 4.0), which permits to copy and distribute the article for non-commercial purposes, provided that the article is not altered or modified and the original author and source are credited. Citation:
Trejo-Aguilar D, Lara-Capistrán L, Maldonado-Mendoza IE, Zulueta-Rodríguez R, Sangabriel-Conde W, Mancera-López M, Negrete-Yankelevich S, Barois I (2013) Loss of arbuscular mycorrhizal fungal diversity in trap cultures during long-term subculturing. IMA Fungus 4(2): 161-167. https://doi.org/10.5598/imafungus.2013.04.02.01 |  |
Abstract
Long-term successional dynamics of an inoculum of arbuscular mycorrhizal fungi (AMF) associated with the maize rhizosphere (from traditionally managed agroecosystems in Los Tuxtlas, Veracruz, Mexico), was followed in Bracchiaria comata trap cultures for almost eight years. The results indicate that AMF diversity is lost following long-term subculturing of a single plant host species. Only the dominant species, Claroideoglomus etunicatum, persisted in pot cultures after 13 cycles. The absence of other morphotypes was demonstrated by an 18S rDNA survey, which confirmed that the sequences present solely belonged to C. etunicatum. Members of Diversisporales were the first to decrease in diversity, and the most persistent species belonged to Glomerales.
Keywords
AM fungi, Diversisporales, Glomerales, preservation, rhizosphere