IMA Fungus 11(1): e33894, doi: 10.1186/s43008-020-00045-9
Naming the untouchable – environmental sequences and niche partitioning as taxonomical evidence in fungi
Anna Rosling‡, Kerri Kluting§, Jeanette Tångrot|, Hector Urbina§, Tea Ammunet§, Shadi Eshghi Sahraei§, Martin Rydén§, Martin Ryberg¶, Anna Rosling#‡ Uppsala University, Uppsala, Sweden§ Uppsala University, Department of Ecology and Genetics, Evolutionary Biology, Uppsala, Sweden| Umeå University, Department of Molecular Biology, National Bioinformatics Infrastructure Sweden (NBIS), SciLifeLab, Umeå, Sweden¶ Uppsala University, Department of Organismal Biology, Systematic Biology, Uppsala, Sweden# Uppsala University, Evolutionary Biology Centre, Uppsala, Sweden
© Anna Rosling, Kerri Kluting, Jeanette Tångrot, Hector Urbina, Tea Ammunet, Shadi Eshghi Sahraei, Martin Rydén, Martin Ryberg, Anna Rosling. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY-NC 4.0), which permits to copy and distribute the article for non-commercial purposes, provided that the article is not altered or modified and the original author and source are credited. Citation:
Rosling A, Kluting K, Tångrot J, Urbina H, Ammunet T, Eshghi Sahraei S, Rydén M, Ryberg M, Rosling A (2020) Naming the untouchable – environmental sequences and niche partitioning as taxonomical evidence in fungi. IMA Fungus 11(1): e33894. https://doi.org/10.1186/s43008-020-00045-9 |  |
Abstract
Due to their submerged and cryptic lifestyle, the vast majority of fungal species are difficult to observe and describe morphologically, and many remain known to science only from sequences detected in environmental samples. The lack of practices to delimit and name most fungal species is a staggering limitation to communication and interpretation of ecology and evolution in kingdom Fungi. Here, we use environmental sequence data as taxonomical evidence and combine phylogenetic and ecological data to generate and test species hypotheses in the class Archaeorhizomycetes (Taphrinomycotina, Ascomycota). Based on environmental amplicon sequencing from a well-studied Swedish pine forest podzol soil, we generate 68 distinct species hypotheses of Archaeorhizomycetes, of which two correspond to the only described species in the class. Nine of the species hypotheses represent 78% of the sequenced Archaeorhizomycetes community, and are supported by long read data that form the backbone for delimiting species hypothesis based on phylogenetic branch lengths.
Keywords
Archaeorhizomyces victor nom. seq., Archaeorhizomyces secundus nom. seq., Biodiversity, Dark matter fungi/dark taxa, Realized niche, Voucherless taxa