IMA Fungus 14(1): e34015, doi: 10.1186/s43008-023-00124-7
Sugarcane: an unexpected habitat for black yeasts in Chaetothyriales
Sybren de Hoog‡, Vânia Aparecida Vicente, Morgana F. Voidaleski§, Renata R. Gomes§, Guilherme F. Reis|, Bruna J. Lima§, Giovanna Z. Candido§, Marlon R. Geraldo§, Jade M. Soares¶, Gabriela X. Schneider§, Edvaldo da Trindade#, Israel H. Bini#, Leandro F. Moreno§, Amanda Bombassaro§, Flávio Queiroz-Telles§, Roberto T. Raittz¤, Yu Quan«, Paulo Arruda», Derlene Attili-Angelis˄, Sybren De Hoog§, Vania A. Vicente|‡ Utrecht, Netherlands§ Federal University of Paraná, Microbiology, Parasitology and Pathology Post-Graduation Program, Department of Basic Pathology, Curitiba, Brazil| Federal University of Paraná, Engineering Bioprocess and Biotechnology Post-Graduation Program, Department of Bioprocess Engineering and Biotechnology, Curitiba, Paraná, Brazil¶ Federal University of Paraná, Biological Sciences Graduation, Curitiba, Paraná, Brazil# Federal University of Paraná, Department of Cell Biology, Curitiba, Paraná, Brazil¤ Federal University of Paraná, Laboratory of Bioinformatics, Professional and Technological Education Sector, Curitiba, Brazil« University Medical Center / Canisius Wilhelmina Hospital, Center of Expertise in Mycology of Radboud, Nijmegen» State University of Campinas (UNICAMP), Molecular Biology and Genetics Engineering Center, Campinas, São Paulo, Brazil˄ State University of Campinas (UNICAMP), Division of Microbial Resources (DRM/CPQBA), Campinas, São Paulo, Brazil
© Sybren de Hoog, Vânia Aparecida Vicente, Morgana Voidaleski, Renata Gomes, Guilherme Reis, Bruna Lima, Giovanna Candido, Marlon Geraldo, Jade Soares, Gabriela Schneider, Edvaldo Trindade, Israel Bini, Leandro Moreno, Amanda Bombassaro, Flávio Queiroz-Telles, Roberto Raittz, Yu Quan, Paulo Arruda, Derlene Attili-Angelis, Sybren De Hoog, Vania Vicente. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY-NC 4.0), which permits to copy and distribute the article for non-commercial purposes, provided that the article is not altered or modified and the original author and source are credited. Citation:
de Hoog S, Vicente VA, Voidaleski MF, Gomes RR, Reis GF, Lima BJ, Candido GZ, Geraldo MR, Soares JM, Schneider GX, Trindade E, Bini IH, Moreno LF, Bombassaro A, Queiroz-Telles F, Raittz RT, Quan Y, Arruda P, Attili-Angelis D, De Hoog S, Vicente VA (2023) Sugarcane: an unexpected habitat for black yeasts in Chaetothyriales. IMA Fungus 14(1): e34015. https://doi.org/10.1186/s43008-023-00124-7 |  |
Abstract
Sugarcane (Saccharum officinarum, Poaceae) is cultivated on a large scale in (sub)tropical regions such as Brazil and has considerable economic value for sugar and biofuel production. The plant is a rich substrate for endo- and epiphytic fungi. Black yeasts in the family Herpotrichiellaceae (Chaetothyriales) are colonizers of human-dominated habitats, particularly those rich in toxins and hydrocarbon pollutants, and may cause severe infections in susceptible human hosts. The present study assessed the diversity of Herpotrichiellaceae associated with sugarcane, using in silico identification and selective isolation. Using metagenomics, we identified 5833 fungal sequences, while 639 black yeast-like isolates were recovered in vitro. In both strategies, the latter fungi were identified as members of the genera Cladophialophora, Exophiala, and Rhinocladiella (Herpotrichiellaceae), Cyphellophora (Cyphellophoraceae), and Knufia (Trichomeriaceae). In addition, we discovered new species of Cladophialophora and Exophiala from sugarcane and its rhizosphere. The first environmental isolation of Cladophialophora bantiana is particularly noteworthy, because this species up to now is exclusively known from the human host where it mostly causes fatal brain disease in otherwise healthy patients.
Keywords
Metagenomics, Selective isolation, Cladophialophora bantiana