IMA Fungus 15(1): e34076, doi: 10.1186/s43008-024-00141-0
Apiospora arundinis, a panoply of carbohydrate-active enzymes and secondary metabolites
expand article infoTrine Sørensen, Celine Petersen, Asmus T. Muurmann‡, J. V. Cristiansen§, Mathias L. Brundtø‡, Christina K. Overgaard‡, Anders T. Boysen‡, Rasmus D. Wollenberg‡, Thomas O. Larsen§, Jens L. Sørensen, Kåre L. Nielsen, Teis E. Sondergaard|
‡ Aalborg University, Department of Chemistry and Bioscience, Aalborg, Denmark§ Technical University of Denmark, Department of Biotechnology and Biomedicine, Kongens Lyngby, Denmark| Aalborg University, Aalborg, Denmark
© Trine Sørensen, Celine Petersen, Asmus Muurmann, J. Cristiansen, Mathias Brundtø, Christina Overgaard, Anders Boysen, Rasmus Wollenberg, Thomas Larsen, Jens L. Sørensen, Kåre L. Nielsen, Teis E. Sondergaard.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY-NC 4.0), which permits to copy and distribute the article for non-commercial purposes, provided that the article is not altered or modified and the original author and source are credited.
Citation: Sørensen T, Petersen C, Muurmann AT, Cristiansen JV, Brundtø ML, Overgaard CK, Boysen AT, Wollenberg RD, Larsen TO, Sørensen JL, Nielsen KL, Sondergaard TE (2024) Apiospora arundinis, a panoply of carbohydrate-active enzymes and secondary metabolites. IMA Fungus 15(1): e34076. https://doi.org/10.1186/s43008-024-00141-0
Open Access
Abstract
The Apiospora genus comprises filamentous fungi with promising potential, though its full capabilities remain undiscovered. In this study, we present the first genome assembly of an Apiospora arundinis isolate, demonstrating a highly complete and contiguous assembly estimated to 48.8 Mb, with an N99 of 3.0 Mb. Our analysis predicted a total of 15,725 genes, with functional annotations for 13,619 of them, revealing a fungus capable of producing very high amounts of carbohydrate-active enzymes (CAZymes) and secondary metabolites. Through transcriptomic analysis, we observed differential gene expression in response to varying growth media, with several genes related to carbohydrate metabolism showing significant upregulation when the fungus was cultivated on a hay-based medium. Finally, our metabolomic analysis unveiled a fungus capable of producing a diverse array of metabolites.
Keywords
Oxford Nanopore sequencing, Apiospora, Arthrinium, CAZymes, Secondary metabolites